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Addgene inc pjr98
Pjr98, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pjr98/CMV-Brainbow-2%2E1+R+(Plasmid+%2318723)/bio_rxiv__2025__11__12__688028-180-8-9
Average 93 stars, based on 20 article reviews
pjr98 - by Bioz Stars, 2026-10
93/100 stars

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Related Articles

Expressing:

Article Title: Comprehensive interrogation of synthetic lethality in the DNA damage response
Article Snippet: .. For dual guide expression vectors, homologies with the backbone and protospacers were added to the insert from pJR98 (Addgene plasmid no. 187239; containing the constant region CR3 for the first sgRNA and hU6 promoter for the second sgRNA) using PCR. ..

Article Title: Comprehensive interrogation of synthetic lethality in the DNA damage response.
Article Snippet: .. For dual guide expression vectors, homologies with the backbone and protospacers were added to the insert from pJR98 (Addgene plasmid no. 187239; containing the constant region CR3 for the first sgRNA and hU6 promoter for the second sgRNA) using PCR. ..

Plasmid Preparation:

Article Title: Comprehensive interrogation of synthetic lethality in the DNA damage response
Article Snippet: .. For dual guide expression vectors, homologies with the backbone and protospacers were added to the insert from pJR98 (Addgene plasmid no. 187239; containing the constant region CR3 for the first sgRNA and hU6 promoter for the second sgRNA) using PCR. ..

Article Title: Comprehensive interrogation of synthetic lethality in the DNA damage response.
Article Snippet: .. For dual guide expression vectors, homologies with the backbone and protospacers were added to the insert from pJR98 (Addgene plasmid no. 187239; containing the constant region CR3 for the first sgRNA and hU6 promoter for the second sgRNA) using PCR. ..

Polymerase Chain Reaction:

Article Title: Comprehensive interrogation of synthetic lethality in the DNA damage response
Article Snippet: .. For dual guide expression vectors, homologies with the backbone and protospacers were added to the insert from pJR98 (Addgene plasmid no. 187239; containing the constant region CR3 for the first sgRNA and hU6 promoter for the second sgRNA) using PCR. ..

Article Title: Comprehensive interrogation of synthetic lethality in the DNA damage response.
Article Snippet: .. For dual guide expression vectors, homologies with the backbone and protospacers were added to the insert from pJR98 (Addgene plasmid no. 187239; containing the constant region CR3 for the first sgRNA and hU6 promoter for the second sgRNA) using PCR. ..

Amplification:

Article Title: Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq
Article Snippet: Guide oligos containing sets of two sgRNA sequences, separated by a spacer region, were ordered from Twist Bioscience, PCR-amplified, and cloned into pJR101 by ligation into the BstXI/BlpI restriction sites. .. The BsmBI-flanked spacer was then replaced by a fragment amplified from pJR98 (Addgene #140096), carrying the constant region of the first sgRNA and the promoter for the second one. ..

Article Title: Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq
Article Snippet: Guide oligos containing sets of two sgRNA sequences, separated by a spacer region, were ordered from Twist Bioscience, PCR-amplified, and cloned into pJR101 by ligation into the BstXI/BlpI restriction sites. .. The BsmBI-flanked spacer was then replaced by a fragment amplified from pJR98 (Addgene #140096), carrying the constant region of the first sgRNA and the promoter for the second one. ..

Sequencing:

Article Title: Epigenetic silencing of transposable elements by IRF2BP2 is a selective dependency of myeloid leukemia
Article Snippet: Complementary dual-sgRNA oligonucleotides containing BsmBI restriction sites were annealed, phosphorylated with T4 PNK (NEB), diluted, and ligated into the digested pJR103 backbone. .. An additional U6 promoter sequence was retrieved from pJR98 (Addgene #187239) using BsmBI-V2 (NEB), and cloned into the BsmBI-V2 site in the double sgRNA-containing pJR103 vectors. .. The plasmid pHR-UCOE-EF1a-ZIM3-dCas9-P2A-GFP (Addgene #188778) was used for the expression of dCas9-ZIM3-KRAB-GFP in CRISPR interference experiments.

Clone Assay:

Article Title: Epigenetic silencing of transposable elements by IRF2BP2 is a selective dependency of myeloid leukemia
Article Snippet: Complementary dual-sgRNA oligonucleotides containing BsmBI restriction sites were annealed, phosphorylated with T4 PNK (NEB), diluted, and ligated into the digested pJR103 backbone. .. An additional U6 promoter sequence was retrieved from pJR98 (Addgene #187239) using BsmBI-V2 (NEB), and cloned into the BsmBI-V2 site in the double sgRNA-containing pJR103 vectors. .. The plasmid pHR-UCOE-EF1a-ZIM3-dCas9-P2A-GFP (Addgene #188778) was used for the expression of dCas9-ZIM3-KRAB-GFP in CRISPR interference experiments.

other:

Article Title: Systematic functional interrogation of SARS-CoV-2 host factors using Perturb-seq.
Article Snippet: Guide oligos containing sets of two sgRNA sequences, separated by a spacer region, were ordered from Twist Bioscience, PCR-amplified, and cloned into pJR101 by ligation into the BstXI/BlpI restriction sites.

Recombinant:

Article Title: Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors
Article Snippet: Recombinant DNA reagent , Plasmid pU6-sgRNA EF1alpha-puro-T2A-mCherry , This paper , Addgene: 188780 , Further information in . .. Recombinant DNA reagent , pJR98 , This paper , Addgene: 187239 , CR3 constant region – hU6 sgRNA promoter flanked by BsmBI sites. Further information in . .. Recombinant DNA reagent , pJR100 , This paper , Addgene: 187240 , Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression. Further information in .



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Image Search Results


Journal: eLife

Article Title: Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors

doi: 10.7554/eLife.81856

Figure Lengend Snippet:

Article Snippet: Second, the library of IBCs were cloned into pJR98 in an arrayed format. pJR98 was digested by AscI and ssDNA oligo donors of the sequence 5’ CTCTTCCTGCCCGACCTTGGGG – reverse complement IBC – CAGCGCCATAGCTGAGTGTAGATTCGAGC – 3’ were cloned into the vector using NEBuilder HiFI DNA Assembly Master Mix (NEB).

Techniques: Stable Transfection, Marker, Flow Cytometry, Recombinant, Plasmid Preparation, Sequencing, Expressing, Purification, Amplification, Transfection, Software, Genome Wide